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KMID : 1144820190250010023
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대한의생명과학회지
2019 Volume.25 No. 1 p.23 ~ p.31
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Estrogen Induces CK2α Activation via Generation of Reactive Oxygen Species
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Jeong Soo-Yeon
Im Suhn-Young
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Abstract
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The protein kinase CK2α (formerly Casein Kinase II) is implicated in tumorigenesis and transformation. However, the mechanisms of CK2α activation in breast cancer have yet to be elucidated. This study investigated the mechanisms of CK2α activation in estrogen signaling. Estrogen increased reactive oxygen species (ROS) production, CK2α activity, and protein expression in estrogen receptor positive (ER+) MCF-7 human breast cancer cells, which were inhibited by the antioxidant N-acetyl-L-cysteine. H2O2 enhanced CK2α activity and protein expression. Human epidermal growth factor (EGF) increased ROS production, CK2α activity and protein expression in EGF receptor 2 (HER2)-overexpressing MCF-7 (MCF-7 HER2) cells, but not in MCF-7 cells. Estrogen induced the phosphorylation of p38 mitogen-activated protein kinase (MAPK). The p38 inhibitor, SB202190, blocked estrogen-induced increases in ROS production, CK2α activity and CK2α protein expression. The data suggest that ROS/p38 MAPK is the key inducer of CK2α activation in response to estrogen or EGF.
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KEYWORD
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Estrogen, CK2α, ROS, P38 MAPK
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